![]() Enhanced off-target prediction accuracy.īreaking CAS is a versatile system for detecting putative sgRNA off-targets in CRISPR/Cas applications. CrispRVariants enables immediate comparison of variant spectra between target locations.ĬRISPOR assists with guide selection in 120 genomes, including plants and many emerging model organisms, and pre-calculated results for all human coding exons as a UCSC Genome Browser track. This toolkit can be easily used to create a variant allele summary plot and accompanying table of counts. ![]() CrispRVariants allows users to detect problems and filter sequences appropriately before estimating mutation efficiency. Individual variant alleles from either traditional Sanger sequencing or high-throughput CRISPR-Cas9 mutagenesis sequencing experiments can be quantified and visualized. ![]() Off spotter helps in the design of optimal "guide" RNAs (gRNAs) by providing several protospacer adjacent motif (PAM) choices, a run-time definition of the seed and of the allowed number of mismatches.ĬrispRVariants resolves and localizes individual mutant alleles with respect to the endonuclease cut site. ![]()
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